Miasis ocular: el misterio es microscópico / Ocular myiasis: the mystery is microscopic

La miasis ocular es la invasión del órgano ocular y anejos por larvas de insectos que produce dolor, ardor, picazón y enrojecimiento uniocular de inicio abrupto acompañado de sensación de cuerpo extraño en movimiento. Las larvas se acantonan y reproducen invadiendo el tejido progresivamente. Mediante un caso clínico, presentamos los procedimientos diagnósticos y terapéuticos a realizar. Se trata de un agricultor de 56 años que acude a urgencias por prurito y sensación de ocupación en el ojo derecho de 24 horas de evolución sin irritación conjuntival, ni alteraciones visuales, ni limitación motora y el test de fluoresceína fue negativo. Tras lavarlo se evidencian helmintos en el espacio palpebral que más tarde serán identificados como larvas de mosca. Con antibiótico local profiláctico, analgésico tópico y revisiones periódicas en Oftalmología para su arrastre manual se consiguió la recuperación completa. No precisó de tratamiento antiparasitario pues no se objetivó lesión o problema ocular. La miasis es una patología infrecuente que suele confundirse con una conjuntivitis alérgica o viral. Las medidas higiénicas son imprescindibles para prevenirlas como son la limpieza del entorno, la buena higiene personal, la provisión de saneamiento básico y educación sanitaria en el medio rural

Ocular myiasis is the infestation of the eye and adnexa by insect larvae that produces pain, burning, itching and uniocular redness of abrupt onset accompanied by a moving foreign body sensation. The larvae settle and reproduce invading the tissue progressively. Through a clinical case, we present the diagnostic and therapeutic procedures to be performed. This is the case of a 56-year-old farmer who goes to the emergency room for pruritus and foreign body sensation in the right eye for 24 hours after onset without conjunctival irritation, visual disturbances, or limited movement, and with negative fluorescein test. After washing, helminths are evident in the palpebral space, whichwill later be identified as fly larvae. With local prophylactic antibiotics, topical analgesics and periodic control visits to Ophthalmology for manual removal, complete recovery was achieved. He did not need antiparasitic treatment because no eye injury or problem was observed. Myiasis is an infrequent pathology that is often confused with allergic or viral conjunctivitis. Hygiene measures are essential to prevent them, such as cleaning the environment, a good personal hygiene, the provision of basic sanitation and health education in rural areas

Comparing Two Automated Techniques for the Primary Screening-Out of Urine Culture.

Urinary tract infection is the most common human infection with a high morbidity. In primary care and hospital services, conventional urine culture is a key part of infection diagnosis but results take at least 24 h. Therefore, a rapid and reliable screening method is still needed to discard negative samples as quickly as possible and to reduce the laboratory workload. In this aspect, this study aims to compare the diagnostic performance between Sysmex UF-1000i and FUS200 systems in comparison to urine culture as the gold standard. From March to June 2016, 1,220 urine samples collected at the clinical microbiology laboratory of the "Miguel Servet" hospital were studied in parallel with both analysers, and some technical features were evaluated to select the ideal equipment. The most balanced cut-off values taking into account bacteria or leukocyte counts were 138 bacteria/µL or 119.8 leukocyte/µL for the UF-1000i (95.3% SE and 70.4% SP), and 5.7 bacteria/µL or 4.3 leukocyte/µL for the FUS200 (95.8% SE and 44.4% SP). The reduction of cultured plates was 37.4% with the FUS200 and 58.3% with the UF-1000i. This study shows that both techniques improve the workflow in the laboratory, but the UF-1000i has the highest specificity at any sensitivity and the FUS200 needs a shorter processing time.

Serotipos de neumococo en nasofaringe de niños preescolares sanos tras la vacunación antineumocócica conjugada heptavalente / Nasopharyngeal carriage of pneumococcal serotypes in healthy pre-school aged children after 7-valent pneumococcal vaccine

Fundamento y objetivo: Tras la introducción de la vacuna neumocócica conjugada heptavalente (VNC-7v) se plantea investigar en nuestro medio las características que influyen en la colonización por serotipos de neumococo en niños preescolares sanos, la distribución de serotipos y su sensibilidad a antimicrobianos.Sujetos y método: Entre febrero de 2008 y enero de 2009 se recogieron muestras nasofaríngeas a niños de entre 2 meses y 5 años de edad que acudían a revisiones del niño sano en 4 centros de atención primaria de la provincia de Zaragoza (España) para cultivo y serotipado. Mediante regresión logística se estudiaron diferentes variables relacionadas con el estado de portador y las resistencias.Resultados: De los 371 niños estudiados, un 30,7% portaban neumococo en la nasofaringe. Con una cobertura de VNC-7v del 66%, factores relacionados con el hecho de ser portador fueron el número de hermanos (odds ratio [OR] 1,44; intervalo de confianza del 95% [IC 95%] 1,05 a 1,97 por cada hermano), estar escolarizado o asistir a guardería (OR 3,99; IC 95% 2,00 a 7,96), y padecer afección leve de vías respiratorias altas en el momento de la toma (OR 1,72; IC 95% 1,02 a 2,90). Solamente correspondían a serotipos incluidos en la vacuna (STV) un 8,7%. Los serotipos no vacunales más frecuentemente aislados fueron 19A, 6A, 15B, 11 y 15A. Se detectaron significativamente más resistencias a antibióticos entre los STV. Conclusiones: Los niños menores de 6 años de nuestro medio portan neumococos más frecuentemente cuando tienen hermanos, están escolarizados o padecen afecciones leves de vías respiratorias altas. Tras la introducción de la vacuna VNC-7v, los STV son casi anecdóticos (8,7%) y los serotipos emergentes presentan mejor sensibilidad a antibióticos (AU)

Background and objective: To determine the characteristics influencing pneumococcal serotype colonization in healthy pre-school aged children, the distribution of serotypes and their antimicrobial susceptibility, after the introduction of pneumococcal 7-valent conjugate vaccine (VNC-7v). Sujetos and methods: Nasopharyngeal samples were collected from children under 6years of age attending well-child examinations in the province of Zaragoza (Spain). Logistic regression was used to study different variables related to the status of the carriers. Results:Of the 371 children studied 30.7% were found to be carriers. With a vaccine coverage rate of 66%, factors related with presence of pneumococcal carriage were found to be the number of siblings (OR 1.44; CI 95% 1.05-1.97 for each sibling), attending a school or child day care centre (OR 3.99; CI 95% 2.00-7.96) and suffering from a minor upper respiratory tract infection (URTI) (OR 1.72; CI 95% 1.02-2.90). Only 8.7% corresponded to VNC-7v serotypes. The most common non VNC-7v serotypes isolated were 19A, 6A, 15B, 11, and 15A. Significantly greater resistance was detected among VNC-7v serotypes. Conclusion: Children in the setting of this study carried pneumococci more commonly when they have siblings, attend school or day care, or suffer from minor URTI. In the VNC-7v vaccine era, VNC-7v serotypes have become rare occurrences (8.7%) and emerging serotypes present better susceptibility to antibiotics (AU)

[Nasopharyngeal carriage of pneumococcal serotypes in healthy pre-school aged children after 7-valent pneumococcal vaccine]. / Serotipos de neumococo en nasofaringe de niños preescolares sanos tras la vacunación antineumocócica conjugada heptavalente.

BACKGROUND AND OBJECTIVE: To determine the characteristics influencing pneumococcal serotype colonization in healthy pre-school aged children, the distribution of serotypes and their antimicrobial susceptibility, after the introduction of pneumococcal 7-valent conjugate vaccine (VNC-7 v). SUJETOS AND METHODS: Nasopharyngeal samples were collected from children under 6 years of age attending well-child examinations in the province of Zaragoza (Spain). Logistic regression was used to study different variables related to the status of the carriers. RESULTS: Of the 371 children studied 30.7% were found to be carriers. With a vaccine coverage rate of 66%, factors related with presence of pneumococcal carriage were found to be the number of siblings (OR 1.44; CI 95% 1.05-1.97 for each sibling), attending a school or child day care centre (OR 3.99; CI 95% 2.00-7.96) and suffering from a minor upper respiratory tract infection (URTI) (OR 1.72; CI 95% 1.02-2.90). Only 8.7% corresponded to VNC-7 v serotypes. The most common non VNC-7 v serotypes isolated were 19A, 6A, 15B, 11, and 15A. Significantly greater resistance was detected among VNC-7 v serotypes. CONCLUSION: Children in the setting of this study carried pneumococci more commonly when they have siblings, attend school or day care, or suffer from minor URTI. In the VNC-7 v vaccine era, VNC-7 v serotypes have become rare occurrences (8.7%) and emerging serotypes present better susceptibility to antibiotics.